【佳学基因检测】MicroRNA-18a 通过HIF-1α 表达调节口腔鳞状细胞癌细胞的转移特性

品牌基因检测在哪里做——时机窗口

开题评估知道《BMC Oral Health》在. 2022 Sep 5;22(1):378.发表了一篇题目为《MicroRNA-18a 通过 HIF-1α 表达调节口腔鳞状细胞癌细胞的转移特性》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Shihyun Kim, Suyeon Park, Ji-Hyeon Oh, Sang Shin Lee, Yoon Lee, Jongho Choi 等完成。促进了肿瘤的正确治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及正确治疗临床研究内容关键词：

HIF-1α,缺氧,入侵,微小RNA,移民,口腔鳞状细胞癌

肿瘤靶向治疗基因检测临床应用结果

口腔鳞状细胞癌转移风险与靶向药物治疗基因检测背景：口腔鳞状细胞癌的快速转移与预后不良和高死亡率相关。然而，总口腔鳞状细胞癌转移的分子机制尚未有效阐明。尽管 microRNA (miRNA) 的失调表达在恶性肿瘤进展中具有关键作用，但 miRNA 在 总口腔鳞状细胞癌进展中的生物学功能仍不清楚。口腔鳞状细胞癌转移风险与靶向药物治疗基因检测研究旨在探讨 miRNA-18a 通过缺氧诱导因子 1α (HIF-1α) 在 总口腔鳞状细胞癌转移调控中的作用。口腔鳞状细胞癌转移风险与靶向药物治疗基因检测方法： miRNA-18a-5p (miRNA-18a) 在 总患者 (n = 39) 和在 总口腔鳞状细胞癌细胞系（例如，YD-10B 和 HSC-2 细胞）中，使用定量实时聚合酶链反应进行分析。使用蛋白质印迹分析用 miRNA-18a 模拟物或与氯化钴组合处理的 总口腔鳞状细胞癌细胞中的 HIF-1α 蛋白表达。采用MTT法、EdU法和Transwell®插入系统分析了总生存期CC细胞的miRNA-18a表达依赖性增殖和侵袭能力。口腔鳞状细胞癌转移风险与靶向药物治疗基因检测结果：总生存期CC组织中miRNA-18a表达明显低于邻近正常组织。在 总口腔鳞状细胞癌细胞系中，HIF-1α 表达通过 miRNA-18a 模拟处理显着降低。此外，与仅用 miRNA-18a 模拟物处理的细胞相比，miRNA-18a 模拟物显着降低 总口腔鳞状细胞癌细胞的迁移和侵袭能力，而在缺氧条件下 HIF-1α 的过表达显着提高了 总口腔鳞状细胞癌细胞的迁移和侵袭能力。口腔鳞状细胞癌转移风险与靶向药物治疗基因检测结论：miRNA-18a对 HIF-1α 表达产生负面影响并抑制 总口腔鳞状细胞癌的转移，从而表明其作为 总口腔鳞状细胞癌抗转移策略的治疗靶点的潜力。缺氧;入侵；微小RNA；移民;口腔鳞状细胞癌。

肿瘤发生与反复转移国际数据库描述：

Background: Rapid metastasis of oral squamous cell carcinoma (OSCC) is associated with a poor prognosis and a high mortality rate. However, the molecular mechanisms underlying OSCC metastasis have not been fully elucidated. Although deregulated expression of microRNA (miRNA) has a crucial role in malignant cancer progression, the biological function of miRNA in OSCC progression remains unclear. This study aimed to investigate the function of miRNA-18a in OSCC metastatic regulation via hypoxia-inducible factor 1α (HIF-1α).Methods: miRNA-18a-5p (miRNA-18a) expressions in patients with OSCC (n = 39) and in OSCC cell lines (e.g., YD-10B and HSC-2 cells) were analyzed using quantitative real-time polymerase chain reaction. HIF-1α protein expressions in OSCC cells treated with miRNA-18a mimics or combined with cobalt chloride were analyzed using western blotting. The miRNA-18a expression-dependent proliferation and invasion abilities of OSCC cells were analyzed using MTT assay, EdU assay, and a Transwell® insert system.Results: miRNA-18a expression was significantly lower in OSCC tissue than in the adjacent normal tissue. In OSCC cell lines, HIF-1α expression was significantly decreased by miRNA-18a mimic treatment. Furthermore, the migration and invasion abilities of OSCC cells were significantly decreased by miRNA-18a mimics and significantly increased by the overexpression of HIF-1α under hypoxic conditions relative to those abilities in cells treated only with miRNA-18a mimics.Conclusions: miRNA-18a negatively affects HIF-1α expression and inhibits the metastasis of OSCC, thereby suggesting its potential as a therapeutic target for antimetastatic strategies in OSCC.Keywords: HIF-1α; Hypoxia; Invasion; MicroRNA; Migration; Oral squamous cell carcinoma.